human proil 1β Search Results


proil 1β  (R&D Systems)
95
R&D Systems proil 1β
Proil 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+proil+1%CE%B2/pm30300855-76-0-12?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
proil 1β - by Bioz Stars, 2026-07
95/100 stars
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human proil 1β  (OriGene)
92
OriGene human proil 1β
The same volume of in vitro synthesized proIL1β and proIL1β[D 100 A] were used and loaded on the gel. Fragments 1, 2 and 3 are labeled. Putative mature in vitro synthesized sea bass IL1β (MS 101 -Q 261 ) has been loaded as control. Numbers on the left indicate the mass of the molecular weight markers in kDa. The double bands corresponding to the highest molecular weight form (fragment 1) suggested cleavage at the C-terminal end of <t>proIL-1β.</t> This interpretation was supported by N-terminal sequencing of fragment 1′ , which revealed that this fragment has the N-terminal sequence of proIL-1β (M 1 ESEMKC). Furthermore, mutation of D 252 results in a proIL-1β protein that no longer yields fragment 1 upon incubation with caspase-1 .
Human Proil 1β, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+proil+1%CE%B2/pmc03511578-104-17-51?v=OriGene
Average 92 stars, based on 1 article reviews
human proil 1β - by Bioz Stars, 2026-07
92/100 stars
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anti proil 1β  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti proil 1β
The same volume of in vitro synthesized proIL1β and proIL1β[D 100 A] were used and loaded on the gel. Fragments 1, 2 and 3 are labeled. Putative mature in vitro synthesized sea bass IL1β (MS 101 -Q 261 ) has been loaded as control. Numbers on the left indicate the mass of the molecular weight markers in kDa. The double bands corresponding to the highest molecular weight form (fragment 1) suggested cleavage at the C-terminal end of <t>proIL-1β.</t> This interpretation was supported by N-terminal sequencing of fragment 1′ , which revealed that this fragment has the N-terminal sequence of proIL-1β (M 1 ESEMKC). Furthermore, mutation of D 252 results in a proIL-1β protein that no longer yields fragment 1 upon incubation with caspase-1 .
Anti Proil 1β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+proil+1%CE%B2/pm24760326-66-19-20?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
anti proil 1β - by Bioz Stars, 2026-07
96/100 stars
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Image Search Results


The same volume of in vitro synthesized proIL1β and proIL1β[D 100 A] were used and loaded on the gel. Fragments 1, 2 and 3 are labeled. Putative mature in vitro synthesized sea bass IL1β (MS 101 -Q 261 ) has been loaded as control. Numbers on the left indicate the mass of the molecular weight markers in kDa. The double bands corresponding to the highest molecular weight form (fragment 1) suggested cleavage at the C-terminal end of proIL-1β. This interpretation was supported by N-terminal sequencing of fragment 1′ , which revealed that this fragment has the N-terminal sequence of proIL-1β (M 1 ESEMKC). Furthermore, mutation of D 252 results in a proIL-1β protein that no longer yields fragment 1 upon incubation with caspase-1 .

Journal: PLoS ONE

Article Title: Caspase-1 and IL-1β Processing in a Teleost Fish

doi: 10.1371/journal.pone.0050450

Figure Lengend Snippet: The same volume of in vitro synthesized proIL1β and proIL1β[D 100 A] were used and loaded on the gel. Fragments 1, 2 and 3 are labeled. Putative mature in vitro synthesized sea bass IL1β (MS 101 -Q 261 ) has been loaded as control. Numbers on the left indicate the mass of the molecular weight markers in kDa. The double bands corresponding to the highest molecular weight form (fragment 1) suggested cleavage at the C-terminal end of proIL-1β. This interpretation was supported by N-terminal sequencing of fragment 1′ , which revealed that this fragment has the N-terminal sequence of proIL-1β (M 1 ESEMKC). Furthermore, mutation of D 252 results in a proIL-1β protein that no longer yields fragment 1 upon incubation with caspase-1 .

Article Snippet: HSpCMV-XL5proIL1β[D 116 A], HSpCMV-XL5proIL1β[D 128 A] and HSpCMV-XL5proIL1β[D 116 A/D 128 A], for producing mutated forms of human proIL-1β in D 116 , D 128 or D 116 /D 128 : each aspartate was mutated to alanine by site-directed mutagenesis using HSpCMV-XL5proIL1β (IL-1β Human cDNA clone (NM_000576.2) in pCMV-XL5 plasmid from Origene (#SC122566)) as template and the primers listed in .

Techniques: In Vitro, Synthesized, Labeling, Molecular Weight, Sequencing, Mutagenesis, Incubation

( A ) Processing of in vitro translated chicken proIL-1β and mutants by sea bass caspase-1. The same volume of in vitro translated chicken proIL-1β, proIL1β[D 77 A], proIL1β[D 80 A] and proIL1β[D 82 A] were loaded on the gel. In vitro translated putative mature chicken IL-1β forms (MI 119 -R 267 , MI 122 -R 267 and MS 81 -R 267 ) were loaded as controls. ( B ) Processing of in vitro translated human proIL-1β and mutants by sea bass caspase-1. ( C ) Processing of in vitro translated human proIL-1β and mutants by human caspase-1. The same volume of in vitro tranlsated proIL-1β , proIL1β[D 116 A], proIL1β[D 128 A] and proIL1β[D 116 A/D 128 A] was loaded on the gel. In vitro translated mature human IL-1β (MA 117 -S 269 ) and human IL-1β form (MS 129 -S 269 ) starting at S 129 , homologue to sea bass S 101 , were loaded as controls. Mature forms are highlighted by arrow heads. Numbers on the left indicate the mass of the molecular weight markers in kDa.

Journal: PLoS ONE

Article Title: Caspase-1 and IL-1β Processing in a Teleost Fish

doi: 10.1371/journal.pone.0050450

Figure Lengend Snippet: ( A ) Processing of in vitro translated chicken proIL-1β and mutants by sea bass caspase-1. The same volume of in vitro translated chicken proIL-1β, proIL1β[D 77 A], proIL1β[D 80 A] and proIL1β[D 82 A] were loaded on the gel. In vitro translated putative mature chicken IL-1β forms (MI 119 -R 267 , MI 122 -R 267 and MS 81 -R 267 ) were loaded as controls. ( B ) Processing of in vitro translated human proIL-1β and mutants by sea bass caspase-1. ( C ) Processing of in vitro translated human proIL-1β and mutants by human caspase-1. The same volume of in vitro tranlsated proIL-1β , proIL1β[D 116 A], proIL1β[D 128 A] and proIL1β[D 116 A/D 128 A] was loaded on the gel. In vitro translated mature human IL-1β (MA 117 -S 269 ) and human IL-1β form (MS 129 -S 269 ) starting at S 129 , homologue to sea bass S 101 , were loaded as controls. Mature forms are highlighted by arrow heads. Numbers on the left indicate the mass of the molecular weight markers in kDa.

Article Snippet: HSpCMV-XL5proIL1β[D 116 A], HSpCMV-XL5proIL1β[D 128 A] and HSpCMV-XL5proIL1β[D 116 A/D 128 A], for producing mutated forms of human proIL-1β in D 116 , D 128 or D 116 /D 128 : each aspartate was mutated to alanine by site-directed mutagenesis using HSpCMV-XL5proIL1β (IL-1β Human cDNA clone (NM_000576.2) in pCMV-XL5 plasmid from Origene (#SC122566)) as template and the primers listed in .

Techniques: In Vitro, Molecular Weight